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State-of-the-Art Microscope Blazes New Trail in Brain Imaging at the ��ý Stiles-Nicholson Brain Institute

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by Bethany Alex | Thursday, Aug 26, 2021

Scientists at ��ý's Stiles-Nicholson Brain Institute are among the first in the state of Florida to

capture 3D images of clusters of identified brain cells in the intact brain using a new, fully

automated, microscope capable of imaging preparations with a razor-thin sheet of laser light.

The microscope – also known as the "Miltenyi UltraMicroscope Blaze" – is a strategic

investment by the Institute to provide advanced imaging opportunities for ��ý neuroscientists

and their trainees.

"The Blaze is the first automated light sheet microscope in South Florida, and one of only two in

the state," said Randy D. Blakely, Ph.D., executive director of ��ý’s Stiles-Nicholson Brain

Institute. "It's really going to accelerate our science and add to the tremendous technical

resources ��ý offers its scientific community. We have been eyeing this technology for some

time, seeing the microscope’s capabilities as transformative, allowing us to look deep into the brain

with molecular precision and circuit level resolution without needing to disrupt the normal

structure of the tissue."

Video of dopamine and norepinephrine neurons in the mouse brain courtesy of Dr. Lorena Areal, Dr. Paula Kurdziel and Dr. Jana Strickler, Managing Director of the Cell Imaging Core.

In short, the new microscope’s large field of illumination – a sheet of light rather than a point of

light typical of other microscopes – allows for intact brains of mice to be imaged down to the

subcellular level at high speed and with neuronal connections intact. Such visualizations are

essential in understanding how diseases impact brain architecture and how global activity

patterns are formed.

"The Blaze has the ability to provide us with a bird's-eye view of whole organs without losing

subcellular details, something biologists have been longing for," said Qi Zhang, Ph.D., Cell

Imaging Core Scientific director and research associate professor for ��ý's College of

Medicine. Dr. Jana Strickler, managing director of the Core, is now training scientists to use the

new instrument and analyze the large datasets it can create. In 2016, the Institute’s commitment

to high-end imaging technologies landed ��ý a prestigious Nikon Center of Excellence.

“The Jupiter campus is home to terrific research facilities, not only at ��ý, but also at the Max

Planck Florida Institute for Neuroscience, well-known for its electron microscopy and super-

resolution imaging resources, and at the nearby Florida home of Scripps Research”, said

Blakely. “With the Blaze, we take a big step forward in understanding the amazing complexity of the

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State-of-the-Art Microscope Blazes New Trail in Brain Imaging at the ����ý Stiles-Nicholson Brain Institute

State-of-the-Art Microscope Blazes New Trail in Brain Imaging at the ��ý Stiles-Nicholson Brain Institute